Journal: Journal of visualized experiments : JoVE

Article Title: Using microarrays to interrogate microenvironmental impact on cellular phenotypes in cancer.

doi: 10.3791/58957

Figure Lengend Snippet: Materials

Article Snippet: Cadherin-6 (CDH6) , R & D Systems , 2715-CA , ECM.

Techniques: Imaging, Inverted Microscopy, Microarray, Electron Microscopy

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: Identification of the CAIX Interactome using the BioID proximal ligation strategy and validation of high confidence proximal CAIX-associating proteins by co-immunoprecipitation. ( a ) Schematic showing the domain structure of the CAIX-BirA* fusion protein used for the BioID studies. SP, signal peptide; PG, proteoglycan-like domain; CA, catalytic domain; TM, transmembrane domain; IC, intracellular domain. ( b ) FACS analysis showing levels of constitutive expression of the CAIX-BirA* fusion protein expressed by MDA-MB-231 cells (MDA-MB-231-CAIX-BirA*) cultured in normoxia compared to levels of endogenous CAIX expression by WT MDA-MB-231 cells cultured in either normoxia or hypoxia. The filled (gray) histograms indicate specific CAIX staining whereas the dotted lined histograms indicate staining by isotype-specific control IgG. ( c ) Image showing exogenous CAIX (red) localized at the cell surface of MDA-MB-231-CAIX-BirA* cells grown in normoxia. Scale bar, 10 μ M . ( d – g ) Co-IP of high confidence proximal CAIX-associating proteins ( d ) ITGB1, ( e ) ITGA2 ( f ) CD98hc and ( g ) MMP14 with exogenous CAIX expressed by MDA-MB-231-CAIX-BirA* cells cultured in normoxia. ( h – j ) Co-IP of ( h ) ITGB1, ( i ) ITGA2 and ( j ) MMP14 with endogenous CAIX expressed by WT MDA-MB-231 cells cultured in either normoxia (N) or hypoxia (H). Isotype-specific IgG was used as a control for co-IPs and data in ( d ) to ( j ) are representative of at least two independent experiments. IgG, immunoglobulin G.

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Ligation, Biomarker Discovery, Immunoprecipitation, Expressing, Cell Culture, Staining, Control, Co-Immunoprecipitation Assay

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: Selected CAIX-BirA*FLAG high-confidence proximal associating proteins identified by BioID in MDA-MB-231 cells

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques:

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX colocalizes with integrins and MMP14 in pseudopodia-like protrusions resembling lamellipodia. ( a ) Images of MDA-MB-231-CAIX-BirA* cells cultured on type 1 collagen in normoxia, showing colocalization (yellow, arrows in merge) of exogenously expressed CAIX (red) with ITGB1, ITGA2, MMP14 and cofilin (each marker in green), but not with FAK (green; arrowheads). Scale bar, 10 μm. ( b ) Images of WT MDA-MB-231 cells grown in either normoxia or hypoxia, showing colocalization of hypoxia-induced CAIX (red) with F-actin (green) in pseudopodia-like protrusions (arrows, merge). Scale bar, 10 μm. ( c ) Images of WT MDA-MB-231 cells cultured on type 1 collagen in hypoxia, showing colocalization of hypoxia-induced CAIX (red) with F-actin, ITGA2 and MMP14 (green) at leading edges of cells (arrows, merge), but not at focal adhesions. Scale bar, 10 μm. ( d and e ) 3D maximum projections of 2D images of MDA-MB-231-CAIX-BirA* cells transfected with GFP and cultured on type 1 collagen in normoxia, showing robust colocalization of CAIX (red) with ( d ) ITGA2 and ( e ) MMP14 (blue) in pseudopodia-like protrusions and depletion of cytoplasmic GFP (green) from these regions. Images for each channel are shown in the upper panels and merged images are shown in the lower panels. Scale bar, 10 μm.

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Cell Culture, Marker, Transfection

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX regulates migration of breast cancer cells. ( a ) Images of wound-induced cell migration by the indicated MDA-MB-231 cells cultured in normoxia. Scale bar, 300 μm. Dashed lines demarcate the wound boundary at t =0 h and yellow shading indicates the cell-free area. ( b ) Quantification of migration by the cells described in a . *** P <0.001. ( c ) Images of wound-induced migration of MDA-MB-231 CAIX-BirA* cells cultured in normoxia and treated with increasing concentrations of U-104. Scale bar, 300 μm. Wound boundaries and cell-free area are demarcated as described in a . ( d ) Quantification of migration by the cells described in c . * P <0.05, *** P <0.001. Data in panels ( b ) and ( d ) show the mean±s.e.m. of technical replicates ( n =16) and are representative of two independent experiments. ( e ) Wound-induced migration of WT MDA-MB-231 cells cultured in normoxia (black bars) or hypoxia (gray bars) and treated with U-104. Data show the mean±s.e.m. of technical replicates ( n =12) and are representative of three independent experiments. ** P <0.01, *** P <0.001. Statistical analysis was performed using Student’s t -test ( b ) or ANOVA ( d , e ).

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Migration, Cell Culture

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX colocalizes with MMP14 at invadopodia and regulates type I collagen degradation. ( a ) Images of a MDA-MB-231-CAIX-BirA* cell (white dashed line shows boundary) cultured on type I collagen in normoxia, identifying a ROI (yellow box) containing invadopodia (arrows) labeled for Cortactin (cyan), CAIX (red) and MMP14 (green). Profile plots showing colocalization of CAIX and MMP14 at cortactin-positive invadopodia (1 and 2) along the indicated lines (insets) are shown at the right. Scale bar, 10 μm. ( b ) Images of an MDA-MB-231-CAIX-BirA* cell (white dashed line shows cell boundary) cultured on DQ type I collagen in normoxia, identifying an ROI (yellow box) containing regions of collagen degradation (1 and 2, arrows) that are labeled for DQ collagen (cyan), CAIX (red) and MMP14 (green). Profile plots showing colocalization of the signals along the indicated lines (insets) are shown to the right. Scale bar, 10 μm. ( c ) Images showing WT MDA-MB-231 cells cultured in hypoxia on fluorescently labeled gelatin (blue, with black areas denoting regions of degradation) and stained for CAIX (green) and markers of invadopodia (red), including cortactin, Tks5 and MMP14 to demonstrate colocalization at mature invadopodia (white arrows; yellow foci). Scale bars, 10 μm (left), 2 μm (right). ( d ) Quantification of DQ collagen degradation by the indicated MDA-MB-231 cell lines grown in normoxia. *** P <0.001. ( e ) Quantitation of degradation of DQ collagen by MDA-MB-231 CAIX KO and CAIX rescue cells cultured in normoxia. * P <0.05. Data in ( d ) and ( e ) show the mean±s.e.m. of technical replicates ( n =5) and are representative of three independent experiments. ( f ) Dose-dependent inhibition of DQ collagen degradation by CAIX rescue cells treated with U-104. Data show the mean±s.e.m. of technical replicates ( n =4) and are representative of two independent experiments. * P <0.05, ** P <0.01. ( g ) Quantification of DQ collagen degradation by WT MDA-MB-231 and CAIX KO cells cultured in hypoxia. Data show the mean±s.e.m. of technical replicates ( n =8) and are representative of three independent experiments. ** P <0.01. ( h and i ) Invasion through Matrigel by highly metastatic MDA-MB-231 LM2-4 breast cancer cells cultured in hypoxia and ( h ) expressing CAIX-targeted (shCAIX) shRNA or ( i ) treated with U-104. Data show the mean±s.e.m. of three independent experiments. *** P <0.001. Western blots of hypoxia-induced CAIX expression are shown as insets.

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Cell Culture, Labeling, Staining, Quantitation Assay, Inhibition, Expressing, shRNA, Western Blot

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX regulates breast cancer cell invasion and metastasis. ( a ) Schematic showing the domain structure of wild type (WT) huCAIX (459 aa) and constructs lacking either the intracellular domain (ΔIC), the extracellular proteoglycan-like domain (ΔPG) or the indicated point mutations in the IC domain. SP, signal peptide; CA, catalytic domain; TM, transmembrane domain. ( b ) Western blot analysis of the levels of expression of the indicated huCAIX constructs by 4T1shCAIX cells cultured in hypoxia. β-actin served as a loading control. ( c ) Analysis of CAIX catalytic activity using the in-cell carbonic anhydrase activity assay. Assays were performed in normoxia in the presence or absence of U-104 (50 μ M ) as indicated. Levels of CAIX catalytic activity were normalized by using the time required to achieve 50% of the total decrease in pH. Data were normalized to the spontaneous rate of reaction in the presence of buffer alone and the activity of cells expressing WT huCAIX was set to 100%. Data show the mean±s.e.m. of technical replicates ( n =3/group) and are representative of three independent experiments * P <0.05, *** P <0.001. ( d ) Invasion through Matrigel by the indicated 4T1 cell lines cultured in hypoxia. Data show the mean±s.e.m. of three independent experiments. * P <0.05, *** P <0.001. ( e ) Invasion through Matrigel by the 4T1shCAIX cell lines expressing WT, ΔIC and ΔPG variants of huCAIX and cultured in hypoxia. * P <0.05, *** P <0.001. ( f ) Invasion through Matrigel by 4T1 sh/WT huCAIX cells cultured in hypoxia and treated with U-104. *** P <0.001. Western blots of hypoxia-induced CAIX expression are shown as insets. For ( d )– ( f ), data show the mean±s.e.m. of three independent experiments. ( g – i ) Analysis of invasion through type 1 collagen by 4T1 sh/WT huCAIX cells cultured in hypoxia and treated with ( g ) U-104 (50 μ M ), ( h ) anti-MMP14 antibody (20 μg/ml) or ( i ) a combination of anti-MMP14 antibody (20 μg/ml) and U-104 (50 μ M ). * P <0.05, ** P <0.01. Data in ( g )–( i ) show the mean±s.e.m. of three independent experiments. ( j ) Analysis of spontaneous lung metastases formed by the indicated 4T1 cell lines following growth of orthotopic breast tumors. Data show the mean±s.e.m. n =6/group. ( k ) Analysis of experimental lung metastases formed by the indicated 4T1 cell lines. Mean±s.e.m. is shown. n =6/group. * P <0.05, ** P <0.01. Statistical analysis was performed using ANOVA ( c , d , e , k ) or Student’s t -test ( f , g , h ).

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Construct, Western Blot, Expressing, Cell Culture, Control, Activity Assay

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX associates with MMP14 through its intracellular domain and potentiates MMP14 activity. ( a ) Co-IP of huCAIX and MMP14 from 4T1shCAIX cells cultured in hypoxia and expressing WT, ΔIC or ΔPG variants of huCAIX. ( b ) Co-IP of huCAIX and MMP14 from 4T1shCAIX cells cultured in hypoxia and expressing WT huCAIX or the T443A point mutant. ( c ) Co-IP of huCAIX and MMP14 from 4T1shCAIX cells cultured in hypoxia and expressing WT or the Y449A point mutant. ( d ) Co-IP of huCAIX and MMP14 from 4T1shCAIX cells grown in hypoxia and expressing WT huCAIX, the S448A point mutant or the ΔPG mutant. ( e ) Co-IP of huCAIX and MMP14 from 4T1shCAIX cells in hypoxia expressing WT huCAIX, the ΔIC truncation or the double point mutant S448A+Y449A. Isotype-specific IgG was used as a control for co-IPs. ( f ) Time-dependent stimulation of MMP14 activity in the presence (red bars) or absence (black bars) of equimolar concentrations of CAIX. Data show the mean±s.e.m. of technical replicates ( n =5) and are representative of two independent experiments. * P <0.05, ** P <0.01. ( g ) Dose-dependent inhibition of CAIX-mediated stimulation of MMP14 activity in the presence of increasing concentrations of U-104. Mean±s.e.m. of technical replicates ( n =5). Data are representative of two independent experiments. *** P <0.001. ( h ) Inhibition of CAIX-mediated stimulation of MMP14 activity in the presence of increasing amounts of D 2 O (heavy water). Mean±s.e.m. of technical replicates ( n =5). Data are representative of three independent experiments. *** P <0.001. Statistical analysis was performed using Student’s t -test ( f ) or ANOVA ( g , h ).

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Activity Assay, Co-Immunoprecipitation Assay, Cell Culture, Expressing, Mutagenesis, Control, Inhibition

Journal: Oncogene

Article Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion

doi: 10.1038/onc.2017.219

Figure Lengend Snippet: CAIX stimulates the activity of MMP14. Model for CAIX-mediated regulation of MMP14 activity. CAIX, which is upregulated in hypoxia, associates through its intracellular domain with MMP14 at functional invadopodia. At these invasive structures, CAIX functions to potentiate MMP14-mediated collagen degradation by directly contributing H + ions required for MMP14 catalytic activity.

Article Snippet: Indicated concentrations of recombinant CAIX (Cat no. 2188-CA, R&D systems) were pre-incubated with activated MMP14 (Recombinant pro-MMP14, Cat.no.

Techniques: Activity Assay, Functional Assay

Journal: Frontiers in Immunology

Article Title: Increased expression of CD36 and CD163 in clear cell renal cell carcinoma suggests an association between lipid transport and an “M2-like” macrophage phenotype

doi: 10.3389/fimmu.2026.1773666

Figure Lengend Snippet: Expression of CD68 and CD163 in ccRCC samples. (A) CD68 was assessed using immunohistochemistry. (B) Expression of CD68 was assessed using the available TCGA data (KIRC dataset). Tumors were grouped by the pathological tumor size (pT). (C) CD163 was assessed using immunohistochemistry. (D) Expression of CD163 was assessed using the available TCGA data (KIRC dataset). Tumors were grouped by the pathological tumor size (pT). Student’s t-test for two groups and one-way ANOVA for four groups; *p<0.05, **p<0.01, ***p<0.001,****p<0.0001. (E) Multiplex immunofluorescence imaging of ccRCC tumor tissues (representative of three patients) depicting CK (cytokeratin), CD163 and CD68 expression. White bar represents 200 μm (left) and 10 μm (right). (F) UMAP depicting clusters of single-cell data showing the expression of CD68 and CD163. Cell type annotations were adopted from the original publication . (G) Fraction of CD68+ cells co-expressing CD163 in RCC tumor tissue macrophage population. Positivity in scRNA-seq for CD68 and CD163 was defined from raw UMI counts as ≥1 UMI per gene (F) . (H) Flow cytometric analysis of CD45 on cells from central and peripheral ccRCC tumor tissue and adjacent kidney. (I) As in (H) , analysis of CD163 on CD68+ cells from central and peripheral ccRCC tumor tissue and adjacent kidney. Values were normalized to kidney controls. Representative histogram with geometric mean fluorescence intensities on the right. One-way ANOVA with Dunnett post-test comparing to kidney. *p<0.05, **p<0.01, ***p<0.001. (J) Data from (I) , tumor periphery, plotted as individual patients, depicting the portion of CD163+ and CD163neg cells.

Article Snippet: Reagents and antibodies used included FcR Blocking Reagent (Cat# 130-059-901), CD36 PE (Cat# 130-110-877), CD147 APC (Cat# 130-124-295), CD8a PE (Cat# 130-117-201), CD45 PE (Cat# 130-113-118), CD68 PE (Cat# 130-128-345), CD163 PE (Cat# 130-127-908), and pan-Cytokeratin APC (Cat# 130-123-091), all from Miltenyi Biotec.

Techniques: Expressing, Immunohistochemistry, Multiplex Assay, Immunofluorescence, Imaging, Single Cell, Fluorescence

Journal: Journal of visualized experiments : JoVE

Article Title: Using microarrays to interrogate microenvironmental impact on cellular phenotypes in cancer.

doi: 10.3791/58957

Figure Lengend Snippet: Materials

Article Snippet: Cadherin-20 (CDH20) , R & D Systems , 5604-CA , ECM.

Techniques: Imaging, Inverted Microscopy, Microarray, Electron Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS

doi: 10.3390/ijms17111820

Figure Lengend Snippet: CA1 is expressed in human spinal cord motor neurons. Images of the normal human spinal cord immune-stained with CA1 or CA2 antibody using the DAB method (brown color) counter-stained with hematoxylin (blue color). The GαCA1 (1:500) and HRP-RbαCA2 (1:500) antibodies were used for this experiment. ( A ) A low magnification image of the ventral horn of spinal cord stained with the CA1 antibody. Two representative motor neurons are indicated by arrows. The white scale bar indicates 0.25 mm; ( B , C ) Higher magnification of spinal cord images stained with the CA1 antibody; ( D , E ) Higher magnification of spinal cord images stained with the CA2 antibody; The black scale bar indicates 50 μm for ( B – E ).

Article Snippet: Both recombinant human CA1 (#2180-CA) and CA2 (#2184-CA) were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS

doi: 10.3390/ijms17111820

Figure Lengend Snippet: CA1 is differentially regulated from CA2 in ALS spinal cord. ( A ) Western blot analysis of the proteins from either the cytosolic (cyto) or microsomal (mv) fractionation extracted from the spinal cords of the control or ALS subjects probed with CA1 (HRP-GαCA1, 1:5000), CA2 (HRP-RbαCA2, 1:5000), SOD1, and PDI antibodies. An equal amount of proteins were used for each lane for either “cyto” or “mv” fraction; ( B ) Quantitative analyses of the differences in the intensities of immune-reactive signals for each protein between the control and ALS groups. All data points were indicated for each group. The dotted horizontal and solid vertical lines in each group represent “Mean ± SD” of the group value. p values are indicated for each graph, and * indicates p < 0.05.

Article Snippet: Both recombinant human CA1 (#2180-CA) and CA2 (#2184-CA) were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Western Blot, Fractionation, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue

doi: 10.3389/fbioe.2026.1772891

Figure Lengend Snippet: Cultivation of highly pure human primary fibroblasts from fibrotic end-stage lungs. (A–F) Fibroblast culture in early and late stages. (A) High-power magnification of a lung tissue cell isolate 24 h after tissue dissociation and seeding. Various adherent (asterisks) and non-adherent (arrows) cell types are visible. (B) High-power magnification of a lung tissue cell isolate 24 h after tissue dissociation and after one wash/medium change. Non-adherent cells (arrows) have been reduced significantly. Fibroblast-like (elongated) phenotypes (spiky arrows) are visible. (C, D) High- (C) and low-power (D) magnification of the cell culture after 7 days. Activated fibroblasts (dagger) that are increased in size start to form cluster. (E) Low-power magnification of the cell culture after 14 days. Fibroblasts (dagger) start to overgrow other cell types (asterisks). (F) Overview of a 21-day-old culture that has been split once. Fibroblasts (dagger) cover approximately 99% of the surface of the culture plate, other cell types are not visible. Scale bars: 100 μm Original magnifications: ×200 (A–C) ; ×100 (D, E) ; ×40 (F) . (G) Gating strategy to validate the purity of fibroblasts in passage 7 before co-culture. Top section: Isotype control: Exemplary sample of primary human fibroblasts incubated with IgG-FITC, IgG-APC and IgG-VioBlue at passage 7. Bottom section: Exemplary sample of primary human fibroblasts stained with anti-CD90 (conjugate: FITC), anti-CD31 (conjugate: APC) and anti-CD45 (conjugate: VioBlue) at passage 7. APC, Allophycocyanin; FITC, Fluorescein isothiocyanate; FSC-A, Forward scatter area; FSC-H, Forward scatter height; IgG, Immunoglobulin G; SSC-A, Side scatter area.

Article Snippet: Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or isotype controls (all from Miltenyi Biotec: Isotype FITC recombinant human IgG1, clone REA293, Cat. No.: 130-118-354; Isotype VioBlue recombinant human IgG1, REA293, Cat. No.: 130-117-362; Isotype APC recombinant human IgG1, REA293, Cat. No.: 130-120-709).

Techniques: Cell Culture, Co-Culture Assay, Control, Incubation, Staining

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: ( A ) Process of formation of successive biosensor layers. Curves I–V presents five different CDH12 concentrations; ( B ) Langmuir curve.

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: Intra-run and inter-run precision and accuracy.

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: Influence of potential interferents on CDH12 assays.

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: Passing–Bablok regression curves comparing the results of the SPRi CDH12 and ELISA methods performed on ( A ) plasma samples and ( B ) peritoneal fluid samples. The blue solid line marks the Passing–Bablok regression curve; the black fine dashed line indicates where the bias would be zero (SPRi CDH12 = ELISA).

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: WHO’s ASSURED criteria for SPRi CDH12 tests.

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques: Clinical Proteomics, Diagnostic Assay, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid

doi: 10.3390/ijms242316894

Figure Lengend Snippet: Schematic representation of the procedures for biosensor preparation, CDH12 quantitative analysis, and biosensor regeneration.

Article Snippet: Monoclonal recombinant rat anti-CDH12 antibody (Cat. No. MAB2240) and recombinant human CDH12 protein (Cat. No. 2240-CA), each >95% pure, were purchased from R&D Systems.

Techniques: